Optimising PHBV biopolymer production in haloarchaea via CRISPRi-mediated redirection of carbon flux.
Lin L, Chen J, Mitra R, Gao Q, Cheng F, Xu T, Zuo Z, Xiang H, Han J
Commun Biol. 2021 Aug 25;4(1):1007. doi: 10.1038/s42003-021-02541-z.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 174382 | pWL502-PAM | Plasmids bearing protospacer sequences with functional PAM efficiently triggered CRISPR-mediated defense in cells with corresponding spacer sequence; article demonstrates use in Haloferax mediterranei |
| 174383 | pWL502-crRNA | The plasmids carrying a mini-CRISPR array were used to express crRNAs; article demonstrates use in Haloferax mediterranei |
| 174384 | pBM23-pyrF-NT | pBM23 contained pyrF and mini-CRISPR. |
| 174385 | pHFX-cas3UD | Plasmids for gene knockout were constructed based on suicide plasmid pHFX |
| 174388 | pMD18-T-pyrF | modified pMD18-T containing pyrF and its native promoter, Ampr |
| 174390 | pHFX-B60-EPSUD | Plasmids for gene knockout were constructed based on suicide plasmid pHFX-B60 |
| 174396 | pBM23-pyrF-CS | pBM23 contained pyrF and mini-CRISPR. |