Rational engineering of a modular bacterial CRISPR-Cas activation platform with expanded target range.
Villegas Kcam MC, Tsong AJ, Chappell J
Nucleic Acids Res. 2021 May 7;49(8):4793-4802. doi: 10.1093/nar/gkab211.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 174366 | pJEC578 | dCas9 for modular CRISPRa. SYNZIP-dCas9. Expresses dCas9 with a SYNZIP18 domain fused to the N terminus. |
| 174367 | pJEC626 | Activation domain for modular CRISPRa. AD-SYNZIP. Expresses alphaNTD from the E coli RNAP with a SYNZIP17 domain fused to the N terminus. |
| 174368 | pJEC625 | Golden gate vector for gRNA cloning. Contains BbsI sites for insertion of the gRNA spacer as annealed oligos. |
| 174369 | pJEC581 | RFP reporter used to evaluate CRISPRa in bacteria, contains gRNA target sites every 10 bp upstream of the promoter. |