The molecular basis of coupling between poly(A)-tail length and translational efficiency.
Xiang K, Bartel DP
Elife. 2021 Jul 2;10. pii: 66493. doi: 10.7554/eLife.66493.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 174337 | C022 | Template for making Rluc mRNA reporter with no pA. |
| 174338 | C031 | Template for making Rluc mRNA reporter with pA30. |
| 174339 | C033 | Template for making Rluc mRNA reporter with pA63. |
| 174340 | C034 | Template for making Rluc mRNA reporter with pA98. |
| 174341 | C035 | Template for making Rluc mRNA reporter with pA120. |
| 174342 | C054 | Template for making Fluc mRNA reporter with pA124 and mutant mouse Malat1. |
| 174343 | C071 | Template for making Nluc mRNA reporter with pA29. |
| 174344 | C093 | Template for making Nluc mRNA reporter with pA63. |
| 174345 | C098 | Template for making Nluc mRNA reporter with pA139. |
| 174346 | C077 | Template for making mRNA for overexpressing frog PABPC1 (C-FLAG) in frog oocytes |
| 174347 | C078 | Template for making mRNA for overexpressing frog ePAB (C-FLAG) in frog oocytes |
| 174348 | C079 | Template for making mRNA for overexpressing eGFP in frog oocytes |
| 174349 | C233 | Template for making mRNA for overexpressing frog PABPC1 M161A mutant (C-FLAG) in frog oocytes |
| 174350 | C150 | Template for making mRNA for overexpressing human eIF4G (C-FLAG) in frog oocytes |
| 174351 | C245 | Template for making mRNA for overexpressing frog RPL3 (C-HA) in frog oocytes |
| 174352 | C255 | Template for making mRNA for overexpressing eGFP (C-FLAG+HA) in frog oocytes |
| 174353 | C064 | Plasmid for expressing frog PABPC1 (C-His) in E.coli |
| 174354 | C119 | CRISPR-Cas9 with guide targeting human PABPC1 genomic location near STOP codon |
| 174355 | C152 | Repair plasmid for introducing AID, T2A and mCherry at the C-teminus of human PABPC1 |
| 174356 | C278 | For introducing multiple copies of OsTIR1 with the piggyBac system |
| 174357 | C194 | For expressing GFP in human cell lines via transfection |
| 174358 | C204 | For expressing siRNA-resistant human PABPC1 in human cell lines via transfection |
| 174359 | C205 | For expressing siRNA-resistant frog ePAB in human cell lines via transfection |
| 174360 | C211 | For expressing siRNA-resistant human PABPC1 mutant (I110L, D111E, D117E, A121G, G139A, Y140F, T147S, R166K) in human cell lines via transfection |