NKD2 mediates stimulation-dependent ORAI1 trafficking to augment Ca(2+) entry in T cells.

Wu B, Woo JS, Vila P, Jew M, Leung J, Sun Z, Srikanth S, Gwack Y
Cell Rep. 2021 Aug 24;36(8):109603. doi: 10.1016/j.celrep.2021.109603. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
174323	pLentiguide-puro_hNKD2sg#1	gRNA targeting hNKD2 - encodes puromycin resistance
174324	pLentiguide-puro_hNKD2sg#2	gRNA targeting hNKD2 - encodes puromycin resistance
174325	pLentiguide-puro_hNKD2sg#3	gRNA targeting hNKD2 - encodes puromycin resistance
174326	pLentiguide-puro_hORAI1sg	gRNA targeting hORAI1 - encodes puromycin resistance
174327	MO70-C-FLAG-hNKD2	encodes human NKD2 cDNA with a C-terminal FLAG tag in retroviral expression vector. Encodes GFP from IRES site and puromycin resistance
174328	pEGFPN1-hNKD2	encodes human NKD2 cDNA with a C-terminal GFP-fusion.
174329	pcDNA3.1_hNKD2	encodes human NKD2 cDNA with a C-terminal MycHis Tag.
174330	FG11F-C-FLAG-hNKD2	encodes human NKD2 cDNA with a C-terminal FLAG tag in lentiviral vector. Does not encode any fluorescence/resistance marker.
174331	pCDNA3.1-NKD2S31A	encodes human NKD2 S31A mutant cDNA with a C-terminal MycHis Tag.
174332	pN1mcherry-NKD2S31A	encodes human NKD2 S31A mutant cDNA with a C-terminal mCherry fusion.
174333	pN1mcherry-NKD2EFMUT	encodes human NKD2 132DFD134>AFA mutant (two D mutated to A)cDNA with a C-terminal mCherry fusion.
174334	pN1mcherry-NKD2G2A	encodes human NKD2 G2A (2nd Gly residue to Ala) mutant cDNA with a C-terminal mCherry fusion.
174335	pEGFPC1-ORAI1-EC-HA	encodes hORAI1 with HA-tag in its extracellular loop 2 and an N-terminal GFP fusion (GFP-ORAI1-EC-HA).
174336	MO91-ORAI1N223A-TEV-pHluorin (ORAI1-EC-pHluorin)	The ORAI1N223A mutant containing-TEV-pHluorin-TEV sequence in the extracellular loop 2
176641	pN1-mCherry-NKD2	Expresses NKD2-mCherry fusion protein in mammalian cells