Robust counterselection and advanced lambdaRed recombineering enable markerless chromosomal integration of large heterologous constructs.
Bubnov DM, Yuzbashev TV, Khozov AA, Melkina OE, Vybornaya TV, Stan GB, Sineoky SP
Nucleic Acids Res. 2022 Aug 26;50(15):8947-8960. doi: 10.1093/nar/gkac649.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 176225 | pRedCm | LambdaRed-expressing plasmid carrying the chloramphenicol acetyltransferase gene repressible by the CI repressor of lambda phage |
| 176226 | pRedCmOcr | A helper plasmid for expression of the artificial T7ocr-gam-bet-exo operon. The plasmid carries the chloramphenicol acetyltransferase gene repressible by the CI repressor of lambda phage. |
| 176227 | pTmpKm | A template plasmid for amplification of the cI-hok-neo cassette |
| 176228 | pTmpSm | A template plasmid for amplification of the cI-hok-strA cassette |
| 176229 | pTmpGm | A template plasmid for amplification of the cI-hok-aacC4 cassette |
| 176230 | pTmpKmH207in | A template plasmid for amplification of the cI-hok-neo cassette, which contains a truncated H207 promoter of T5 phage. The promoter is directed toward the cI gene. |
| 176231 | pTmpKmH207out | A template plasmid for amplification of the cI-hok-neo cassette, which contains a truncated H207 promoter of T5 phage. The promoter is directed outward the cI gene. |
| 176232 | pTmpKmA1O4in | A template plasmid for amplification of the cI-hok-neo cassette, which contains A1O4 promoter. The promoter is directed toward the cI gene. |
| 176233 | pTmpKmA1O4out | A template plasmid for amplification of the cI-hok-neo cassette, which contains LacI-repressible A1O4 promoter. The promoter is directed outward the cI gene. |