The auxin-inducible degron 2 (AID2) system enables controlled protein knockdown during embryogenesis and development in Caenorhabditis elegans.
Negishi T, Kitagawa S, Horii N, Tanaka Y, Haruta N, Sugimoto A, Sawa H, Hayashi KI, Harata M, Kanemaki MT
Genetics. 2021 Dec 2. pii: 6448232. doi: 10.1093/genetics/iyab218.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 174212 | pTN4 | Donor plasmid for introducing Peft-3::AtTIR1(F79G)::mRuby at the ttTi5605 locus |
| 174213 | pGFPmAID | GFP::mAID donor backbone |
| 174214 | pGFPmAID_htz-1 | Donor plasmid for tagging htz-1 with GFP::mAID |
| 174546 | pTK73_htz-1 | htz-1 targeting gRNA expression |
| 178945 | pTN14 | Expresses AtTIR1(F79G) in C elegans |