Selenocysteine substitutions in thiyl radical enzymes.
Caceres JC, Bailey CA, Yokoyama K, Greene BL
Methods Enzymol. 2022;662:119-141. doi: 10.1016/bs.mie.2021.10.014. Epub 2021 Dec 7.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 174210 | pCm N-term SMT3 NikJC199U | Expression plasmid with a TEV removable C-term 8xHis Tag, an N-term SUMO tag and Chloramphenicol resistance. NikJ C199U cloned in the cloning site. |
| 174361 | pCm No carrier NikJC199U | Expression plasmid with a TEV removable C-term 8xHis Tag and Chloramphenicol resistance. NikJ C199U cloned in the cloning site. |
| 174362 | pCm C-term SMT3 NikJC199U | Expression plasmid with a TEV removable C-term 8xHis Tagged SUMO tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site. |
| 174363 | pCm N-term MBP SMT3 NikJC199U | Expression plasmid with a ULP1 removable N-term MBP SUMO tag, a TEV removable C-term 8xHis tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site. |
| 174364 | pCm N-term MBP NikJC199U | Expression plasmid with a TEV removable N-term MBP tag, a TEV removable C-term 8xHis tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site. |
| 174365 | pCm C-term MBP NikJC199U | Expression plasmid with a TEV removable C-term 8xHis tagged MBP tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site. |