Dynamic imaging of nascent RNA reveals general principles of transcription dynamics and stochastic splice site selection.
Wan Y, Anastasakis DG, Rodriguez J, Palangat M, Gudla P, Zaki G, Tandon M, Pegoraro G, Chow CC, Hafner M, Larson DR
Cell. 2021 May 27;184(11):2878-2895.e20. doi: 10.1016/j.cell.2021.04.012. Epub 2021 May 11.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 174198 | Gene Trap - 24XMS2 Lentiviral Vector | Lentiviral expression vector coding for Blasticidin, iRFP and 24X MS2 hairpins. All inserts are cloned in on the -strand to allow for splicing in when targeted into an intron of transcribed genes. |
| 174199 | Gene Trap - 24XPP7 Lentiviral Vector | Lentiviral expression vector coding for Blasticidin, iRFP and 24X PP7 hairpins. All inserts are cloned in on the -strand to allow for splicing in when targeted into an intron of transcribed genes. |