Genetic encoding of a highly photostable, long lifetime fluorescent amino acid for imaging in mammalian cells.
Jones CM, Robkis DM, Blizzard RJ, Munari M, Venkatesh Y, Mihaila TS, Eddins AJ, Mehl RA, Zagotta WN, Gordon SE, Petersson EJ
Chem Sci. 2021 Aug 3;12(36):11955-11964. doi: 10.1039/d1sc01914g. eCollection 2021 Sep 22.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 174099 | pDule-Acd82 | Acridone82 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli. |
| 174100 | pDule2-Acd82 | Acridone82 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli. |
| 174101 | pAcBac1.tR4-AcdRS82 | Expression of orthogonal Mb tRNA and Acd82 tRNA synthetase for incorporation of acridone in eukaryotic cells. |