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Benjamin Kleinstiver Lab: CRISPR-Cas9 vectors expressing mScarlet

Unpublished

Plasmids from Article

ID Plasmid Purpose
174091pCMV-T7-SpCas9-BPNLS(SV40)-3xFLAG-P2A-mScarlet (MNW001)CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a C-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
174136pCMV-T7-SpG-P2A-mScarlet (MNW006)CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
174137pCMV-T7-SpRY-P2A-mScarlet (MNW002)CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R); C-terminal bi-partite NLS, 3x flag tag, P2A-mScarlet
174138pCMV-T7-SpCas9-VRER-P2A-mScarlet (MNW003)CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRER(D1135V/G1218R/R1335E/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
174139pCMV-T7-SpCas9-VRQR-P2A-mScarlet (MNW004)CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRQR(D1135V/G1218R/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
174140pCMV-T7-SpCas9-NG-P2A-mScarlet (MNW005)CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

Antibodies from Article