Mapping the dynamic transfer functions of eukaryotic gene regulation.
Lee JB, Caywood LM, Lo JY, Levering N, Keung AJ
Cell Syst. 2021 Aug 24. pii: S2405-4712(21)00291-X. doi: 10.1016/j.cels.2021.08.003.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 174063 | pJL29 | Expresses for mCherry for S. cerevisiae. Contain 2 43-8 zinc-finger operators upstream of Cyc1 promoter. |
| 174064 | pJL30 | Encodes for 43-8 ZF fused to Cry2. Used for optogenetic recruitment of TF. Used with pJL32. |
| 174066 | pJL32 | Encodes for CIB1 fused to VP16 in S. cerevisiae. Part of optogenetic system. |
| 174067 | pJL45 | Expresses CIB1 in S. cerevisiae. Includes inducible lac operon. |
| 174068 | pJL50 | Expresses 97-3 zinc finger array fused to VP16 in S. cerevisiae. Parent plasmid for chromatin regulator library. |
| 174069 | pJL15 | Expresses 43-8 zinc finger array fused to CRY2 in S. cerevisiae. Part of optogenetic system. |
| 174070 | pJL1 | Expresses VP16-CIB1 in S. cerevisiae. Part of optogenetic system. |