Mapping the dynamic transfer functions of eukaryotic gene regulation.

Lee JB, Caywood LM, Lo JY, Levering N, Keung AJ
Cell Syst. 2021 Aug 24. pii: S2405-4712(21)00291-X. doi: 10.1016/j.cels.2021.08.003. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
174070	pJL1	Expresses VP16-CIB1 in S. cerevisiae. Part of optogenetic system.
174069	pJL15	Expresses 43-8 zinc finger array fused to CRY2 in S. cerevisiae. Part of optogenetic system.
174068	pJL50	Expresses 97-3 zinc finger array fused to VP16 in S. cerevisiae. Parent plasmid for chromatin regulator library.
174067	pJL45	Expresses CIB1 in S. cerevisiae. Includes inducible lac operon.
174066	pJL32	Encodes for CIB1 fused to VP16 in S. cerevisiae. Part of optogenetic system.
174064	pJL30	Encodes for 43-8 ZF fused to Cry2. Used for optogenetic recruitment of TF. Used with pJL32.
174063	pJL29	Expresses for mCherry for S. cerevisiae. Contain 2 43-8 zinc-finger operators upstream of Cyc1 promoter.