Orthogonal CRISPR-Cas tools for genome editing, inhibition, and CRISPR recording in zebrafish embryos.
Takasugi PR, Wang S, Truong KT, Drage EP, Kanishka SN, Higbee MA, Bamidele N, Ojelabi O, Sontheimer EJ, Gagnon JA
Genetics. 2021 Nov 4. pii: 6420709. doi: 10.1093/genetics/iyab196.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 174033 | pCS2-AcrIIA2 | In vitro transcription of anti-CRISPR AcrIIA2 mRNA |
| 174034 | pCS2-AcrIIA4 | In vitro transcription of anti-CRISPR AcrIIA4 mRNA |
| 174035 | pCS2-AcrIIC1 | In vitro transcription of anti-CRISPR AcrIIC1 mRNA |
| 174036 | pCS2-AcrIIC3 | In vitro transcription of anti-CRISPR AcrIIC3 mRNA |
| 174037 | pTol2-HybridBarcode | For Tol2 transgenesis of expressed barcode containing SpyCas9, SauCas9, and LbCas12a target sites |
| 174040 | pCS2-SauCas9 | In vitro transcription of SauCas9 mRNA |