Visual detection of binary, ternary and quaternary protein interactions in fission yeast using a Pil1 co-tethering assay.
Yu ZQ, Liu XM, Zhao D, Xu DD, Du LL
J Cell Sci. 2021 Oct 1;134(19):jcs258774. doi: 10.1242/jcs.258774. Epub 2021 Oct 12.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 174012 | pDB4924 | pDUAL plasmid expressing Pil1-mCherry from 41nmt1 promoter |
| 174013 | pDB1751 | pDUAL plasmid expressing GFP from 41nmt1 promoter |
| 174014 | pDB4958 | pHIS3H plasmid expressing CFP-Atg6 from 41nmt1 promoter |
| 174015 | pDB4961 | pHIS3H plasmid expressing 13Myc-Vps15 from 41nmt1 promoter |
| 174016 | pDB4925 | pDUAL plasmid expressing Pil1-mCherry-Atg38(161-190) from 41nmt1 promoter |
| 174017 | pDB4658 | pDUAL plasmid expressing GFP-Atg8 from 41nmt1 promoter |
| 174018 | pDB4946 | pDUAL plasmid expressing Pil1-mCherry-Atg14 from 41nmt1 promoter |
| 174019 | pDB4948 | pDUAL plasmid expressing Pil1-mCherry-Vps38 from 41nmt1 promoter |
| 174020 | pDB4953 | pDUAL plasmid expressing GFP-Atg6 from 41nmt1 promoter |