Hypercompact CRISPR-Cas12j2 (CasPhi) enables genome editing, gene activation, and epigenome editing in plants.
Liu S, Sretenovic S, Fan T, Cheng Y, Li G, Qi A, Tang X, Xu Y, Guo W, Zhong Z, He Y, Liang Y, Han Q, Zheng X, Gu X, Qi Y, Zhang Y
Plant Commun. 2022 Sep 20:100453. doi: 10.1016/j.xplc.2022.100453.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 173924 | pYPQ-Cas12j2 | Gateway entry plasmid (attL1 & attR5) expressing NLS-Cas12j2-NLS |
| 173926 | pYPQ141-ZmUbi-RZ-Cas12j2 | Gateway entry clone (attL5 & attL2) for Cas12j2 crRNA expression under ZmUbi promoter with ribozyme processing |
| 173927 | pYPQ131-RZ-Cas12j2 | Golden Gate entry vector; empty vector to clone the 1st Cas12j2 crRNA with ribozyme processing |
| 173928 | pYPQ132-RZ-Cas12j2 | Golden Gate entry vector; empty vector to clone the 2nd Cas12j2 crRNA with ribozyme processing |
| 173929 | pYPQ133-RZ-Cas12j2 | Golden Gate entry vector; empty vector to clone the 3rd Cas12j2 crRNA with ribozyme processing |
| 173930 | pYPQ134-RZ-Cas12j2 | Golden Gate entry vector; empty vector to clone the 4th Cas12j2 crRNA with ribozyme processing |
| 189780 | pYPQCas12j2 | Gateway entry plasmid (attL1 & attR5) expressing NLS-Cas12j2-NLS, without promoter |
| 189781 | pYPQ141-ZmUBI-RZ-Cas12j2 | Cas12j2 (CasΦ) Gateway gRNA entry plasmid using Zea mays Ubi promoter and HH, HDV ribozyme processing |
| 189782 | pYPQ131-RZ-Cas12j2 | Golden Gate entry vector to clone the 1st Cas12j2(CasΦ) crRNA flanked by HH and HDV ribozymes |
| 189783 | pYPQ132-RZ-Cas12j2 | Golden Gate entry vector to clone the 2nd Cas12j2(CasΦ) crRNA flanked by HH and HDV ribozymes |
| 189784 | pYPQ133-RZ-Cas12j2 | Golden Gate entry vector to clone the 3rd Cas12j2(CasΦ) crRNA flanked by HH and HDV ribozymes |
| 189785 | pYPQ134-RZ-Cas12j2 | Golden Gate entry vector to clone the 4th Cas12j2(CasΦ) crRNA flanked by HH and HDV ribozymes |