Insights into animal septins using recombinant human septin octamers with distinct SEPT9 isoforms.
Iv F, Martins CS, Castro-Linares G, Taveneau C, Barbier P, Verdier-Pinard P, Camoin L, Audebert S, Tsai FC, Ramond L, Llewellyn A, Belhabib M, Nakazawa K, Di Cicco A, Vincentelli R, Wenger J, Cabantous S, Koenderink GH, Bertin A, Mavrakis M
J Cell Sci. 2021 Jul 8. pii: 270842. doi: 10.1242/jcs.258484.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 174491 | pnEA-vH_His-TEV-SEPT2 | bacterial expression of human SEPT2 |
| 174492 | pnEA-vH_His-TEV-SEPT2-msfGFP | bacterial expression of human SEPT2 fused to monomeric superfolder GFP |
| 174494 | pnEA-vH_His-TEV-DSep1-msfGFP | bacterial expression of Drosophila Sep1 fused to monomeric superfolder GFP |
| 174495 | pnCS_DSep2_Peanut-TEV-Strep | bacterial co-expression of Drosophila Sep2 and of Drosophila Peanut |
| 174497 | pnEA-vH_His-TEV-SEPT2_SEPT6 | bacterial co-expression of human SEPT2 and of human SEPT6 |
| 174498 | pnEA-vH_His-TEV-SEPT2-msfGFP_SEPT6 | bacterial co-expression of human SEPT2 fused to monomeric superfolder GFP and of human SEPT6 |
| 174499 | pnCS_SEPT6_SEPT7-TEV-Strep | bacterial co-expression of human SEPT6 and of human SEPT7 |
| 174500 | pnCS_SEPT7_SEPT9_i1-TEV-Strep | bacterial co-expression of human SEPT7 and of human SEPT9_i1 |
| 174501 | pnCS_SEPT7_SEPT9_i3-TEV-Strep | bacterial co-expression of human SEPT7 and of human SEPT9_i3 |
| 174502 | pnCS_SEPT7_SEPT9_i5-TEV-Strep | bacterial co-expression of human SEPT7 and of human SEPT9_i5 |