Biosynthesis and genetic encoding of phosphothreonine through parallel selection and deep sequencing.
Zhang MS, Brunner SF, Huguenin-Dezot N, Liang AD, Schmied WH, Rogerson DT, Chin JW
Nat Methods. 2017 Jul;14(7):729-736. doi: 10.1038/nmeth.4302. Epub 2017 May 29.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
173899 | pUC_pThrRS_SeptRNAv2.0CUA_OXB20-PduX_EFSep | The pThr/tRNAv2.0CUA pair enables the biosynthesis of proteins containing pThr |