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Optogenetic control of small GTPases reveals RhoA mediates intracellular calcium signaling.

Inaba H, Miao Q, Nakata T
J Biol Chem. 2021 Jan-Jun;296:100290. doi: 10.1016/j.jbc.2021.100290. Epub 2021 Jan 13. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
173863mVenus-NES-SspB-LARG-DH-P2A-iLIDcaaxMammalian expression of mVenus-NES-SspB-LARG-DH-P2A-iLIDcaax (opto-RhoA)
173864mVenus-NES-SspB-MCS-P2A-iLIDcaaxMammalian expression of mVenus-NES-SspB-P2A-iLIDcaax (opto-control) containing multiple cloning sites (MCS)
173865mVenus-NES-SspB-Tiam-DHPH-P2A-iLIDcaaxMammalian expression of mVenus-NES-SspB-Tiam-DHPH-P2A-iLIDcaax (opto-Rac1)
173866mVenus-NES-SspB-ITSN1-DHPH-P2A-iLIDcaaxMammalian expression of mVenus-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax (opto-Cdc42)
173867mVenus-NES-SspB-SOS2cat-P2A-iLIDcaaxMammalian expression of mVenus-NES-SspB-SOS2cat-P2A-iLIDcaax (opto-Ras)
173868mVenus-NES-SspB-C3Gcat-P2A-iLIDcaaxMammalian expression of mVenus-NES-SspB-C3Gcat-P2A-iLIDcaax (opto-Rap)
173869mCherry-NES-SspB-MCS-P2A-iLIDcaaxMammalian expression of mCherry-NES-SspB-P2A-iLIDcaax (opto-control) containing multiple cloning sites (MCS)
173870mCherry-NES-SspB-LARG-DH-P2A-iLIDcaaxMammalian expression of mCherry-NES-SspB-LARG-DH-P2A-iLIDcaax (opto-RhoA)
173871mCherry-NES-SspB-Tiam-DHPH-P2A-iLIDcaaxMammalian expression of mCherry-NES-SspB-Tiam-DHPH-P2A-iLIDcaax (opto-Rac1)
173872mCherry-NES-SspB-ITSN1-DHPH-P2A-iLIDcaaxMammalian expression of mCherry-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax (opto-Cdc42)
173873mCherry-NES-SspB-SOS2cat-P2A-iLIDcaaxMammalian expression of mCherry-NES-SspB-SOS2cat-P2A-iLIDcaax (opto-Ras)
173874mCherry-NES-SspB-C3Gcat-P2A-iLIDcaaxMammalian expression of mVenus-NES-SspB-C3Gcat-P2A-iLIDcaax (opto-Rap)
173875mVenus-NES-SspB-RGL2-GEF-P2A-iLIDcaaxMammalian expression of mVenus-NES-SspB-RGL2-GEF-P2A-iLIDcaax (opto-Ral)
173876mCherry-NES-SspB-RGL2-GEF-P2A-iLIDcaaxMammalian expression of mCherry-NES-SspB-RGL2-GEF-P2A-iLIDcaax (opto-Ral)

Antibodies from Article