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Catalytic turnover-based phage selection for engineering the substrate specificity of Sfp phosphopantetheinyl transferase.

Sunbul M, Marshall NJ, Zou Y, Zhang K, Yin J
J Mol Biol. 2009 Apr 10;387(4):883-98. doi: 10.1016/j.jmb.2009.02.010. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
173491pET-Sfp R4-4 mutantExpression plasmid for mutant R4-4 of Sfp phosphopantetheinyl transferase. R4-4 can use 3′-dephospho CoA for adding the phosphopantetheinyl group to the carrier proteins.

Antibodies from Article