High-Throughput Analysis of Protein Turnover with Tandem Fluorescent Protein Timers.
Fung JJ, Blocher-Juarez K, Khmelinskii A
Methods Mol Biol. 2022;2378:85-100. doi: 10.1007/978-1-0716-1732-8_6.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 173453 | pFA6a-mCherry-mNeonGreen-kanMX (pKBJ006) | Contains mCherry-mNeonGreen tandem fluorescent protein timer |
| 173454 | pFA6a-mScarlet-I-mNeonGreen-kanMX (pKBJ007) | Contains mScarletI-mNeonGreen tandem fluorescent protein timer |
| 173455 | pFA6a-mScarlet-mNeonGreen-kanMX (pKBJ008) | Contains mScarlet-mNeonGreen tandem fluorescent protein timer |
| 173456 | pFA6a-mScarlet-H-mNeonGreen-kanMX (pKBJ009) | Contains mScarletH-mNeonGreen tandem fluorescent protein timer |
| 173457 | pRS41K-mCherry-sfGFP (pJJF001) | C-SWAT donor for Seamless tagging, contains mCherry-sfGFP tandem fluorescent protein timer |
| 173458 | pRS41K-mCherry-sfGFP-ADH1term-TEFpr-hphΔC (pJJF002) | C-SWAT donor for Selection Reconstitution tagging, contains mCherry-sfGFP tandem fluorescent protein timer |
| 173459 | pRS41K-mCherry-mNeonGreen (pJJF003) | C-SWAT donor for Seamless tagging, contains mCherry-mNeonGreen tandem fluorescent protein timer |
| 173460 | pRS41K-mCherry-mNeonGreen-ADH1term-TEFpr-hphΔC (pJJF004) | C-SWAT donor for Selection Reconstitution tagging, contains mCherry-mNeonGreen tandem fluorescent protein timer |
| 173461 | pRS41K-mScarlet-I-mNeonGreen (pKBJ010) | C-SWAT donor for Seamless tagging, contains mScarletI-mNeonGreen tandem fluorescent protein timer |
| 173462 | pRS41K-mScarlet-I-mNeonGreen-ADH1term-TEFpr-hphΔC (pKBJ011) | C-SWAT donor for Selection Reconstitution tagging, contains mScarletI-mNeonGreen tandem fluorescent protein timer |