CRISPR library screening to develop HEK293-derived cell lines with improved lentiviral vector titers.
Iaffaldano BJ, Marino MP, Reiser J
Front Genome Ed. 2023 Jul 13;5:1218328. doi: 10.3389/fgeed.2023.1218328. eCollection 2023.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 173428 | lenticrispr-wt-ltr-puro | Expresses Cas9 and guide RNA, contains intact LTR |
| 182635 | lentiguide-puro-wt-ltr | Expresses guide RNA, contains intact LTR |
| 182646 | pxpr_050-wt-ltr | lentiviral expression of gRNA scaffold, contains intact LTR |
| 182647 | pxpr_502-wt-ltr | for CRISPRa, lentiviral expression of gRNA scaffold using activator P65 HSF, contains intact LTR |