Constructing Cell-Free Expression Systems for Low-Cost Access.
Guzman-Chavez F, Arce A, Adhikari A, Vadhin S, Pedroza-Garcia JA, Gandini C, Ajioka JW, Molloy J, Sanchez-Nieto S, Varner JD, Federici F, Haseloff J
ACS Synth Biol. 2022 Mar 18;11(3):1114-1128. doi: 10.1021/acssynbio.1c00342. Epub 2022 Mar 8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 173224 | pT7-RiboJ-LacZa-T7 | pFGC-T7-RJBB to clone genes by Golden Gate (BsaI). To be used in cell-free systems |
| 173225 | pT7-RiboJ-mTFP1-T7 | Encodes mTFP fluorescent protein. To be used in cell-free system |
| 173226 | pFGC-T7RibJ-RRvT | Encodes RRvT fluorescent protein. To be used in cell-free system |
| 173227 | pT7-RiboJ-mScarlet-T7 | Encodes mScarlet fluorescent protein. To be used in cell-free system |