Marker-free co-selection for successive rounds of prime editing in human cells.

Levesque S, Mayorga D, Fiset JP, Goupil C, Duringer A, Loiselle A, Bouchard E, Agudelo D, Doyon Y
Nat Commun. 2022 Oct 7;13(1):5909. doi: 10.1038/s41467-022-33669-z. PubMed Article

Plasmids from Article

Showing 1 to 10 of 42 entries

Show 102550100All entries

Search table:

• Previous
• 1
• 2
• 3
• 4
• 5
• Next

ID	Plasmid	Purpose
187459	ATP1A1_G4_Q118R_Std_BFP-to-EGFP_Dual_epegRNA_tevopreQ1	Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 pegRNA and EBFP_To_EGFP epegRNA (tevopreq1) from two independent U6 promoters.
187458	ATP1A1_G6_T804N_v1_Dual_epegRNA_tevopreQ1	Coselection for prime editing in human cells. Vector for tandem expression of ATP1A1 T804N-G6 epegRNA (tevopreQ1) with a user-specified epegRNA. pU6-tevopreq1-GG-acceptor-like plasmid
187457	ATP1A1_G6_T804N_Std_Dual_epegRNA_tevopreQ1	Coselection for prime editing in human cells. Vector for tandem expression of ATP1A1 T804N-G6 pegRNA with a user-specified epegRNA. pU6-tevopreq1-GG-acceptor-like plasmid
187456	ATP1A1_G4_Q118R_v2_Dual_epegRNA_tevopreQ1	Coselection for prime editing in human cells. Vector for tandem expression of ATP1A1 Q118R-G4_v2 optimized epegRNA with a user-specified epegRNA. pU6-tevopreq1-GG-acceptor-like plasmid
187455	ATP1A1_T804N_hPGK1_EBFP_Donor	HDR donor plasmid to introduce the T804N mutation conferring cellular resistance to ouabain and the EBFP cassette to ATP1A1 intron 17.
187454	ATP1A1_G4_Q118R_Std_Dual_epegRNA_tevopreQ1	Coselection for prime editing in human cells. Vector for tandem expression of ATP1A1 Q118R-G4 pegRNA with a user-specified epegRNA. pU6-tevopreq1-GG-acceptor-like plasmid
178110	ATP1A1_G6_T804N_MTOR_E2419K_Dual_pegRNA	Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 T804N-G6 and MTOR-E2419K pegRNAs from two independent U6 promoters.
178109	ATP1A1_G6_T804N_MTOR_F2108L_Dual_pegRNA	Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 T804N-G6 and MTOR-F2108L pegRNAs from two independent U6 promoters.
178108	ATP1A1_G6_T804N_MTOR_I2017T_Dual_pegRNA	Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 T804N-G6 and MTOR-I2017T pegRNAs from two independent U6 promoters.
178107	ATP1A1_G8_MTOR_Nick_Exon-53_Dual_sgRNA	Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G8 and MTOR Nick exon-53 sgRNAs from two independent U6 promoters.