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A bacterial three-hybrid assay detects Escherichia coli Hfq-sRNA interactions in vivo.

Berry KE, Hochschild A
Nucleic Acids Res. 2018 Jan 25;46(2):e12. doi: 10.1093/nar/gkx1086. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
174661pKB817 (pBra-Hfq)Plpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli Hfq. Useful as three-hybrid positive control.

Antibodies from Article