Reprogrammed CRISPR-Cas13b suppresses SARS-CoV-2 replication and circumvents its mutational escape through mismatch tolerance.
Fareh M, Zhao W, Hu W, Casan JML, Kumar A, Symons J, Zerbato JM, Fong D, Voskoboinik I, Ekert PG, Rudraraju R, Purcell DFJ, Lewin SR, Trapani JA
Nat Commun. 2021 Jul 13;12(1):4270. doi: 10.1038/s41467-021-24577-9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 173029 | Ef1a-pspCas13b-NES-3xFLAG-T2A-BFP | For expression of cytoplasmic pspCas13b protein tagged with 3xHA-T2A-BFP for gene silencing in mamallian cells. |
| 173030 | SARS-CoV-2 Nucleocapsid-3xHA-IRES-mcherry | To express SARS-CoV-2 Nucleocapsid protein tagged with 3xHA and in frame with mcherry (NCP-IRES-mcherry) in mammalian cells |