FluoSTEPs: Fluorescent biosensors for monitoring compartmentalized signaling within endogenous microdomains.
Tenner B, Zhang JZ, Kwon Y, Pessino V, Feng S, Huang B, Mehta S, Zhang J
Sci Adv. 2021 May 21;7(21). pii: 7/21/eabe4091. doi: 10.1126/sciadv.abe4091. Print 2021 May.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 181856 | pcDNA3.1(+)-FluoSTEP-AktAR | Genetically encoded FRET-based sensor for monitoring Akt activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence. |
| 181857 | pcDNA3.1(+)-FluoSTEP-JNKAR | Genetically encoded FRET-based sensor for monitoring JNK kinase activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence. |
| 181858 | pcDNA3.1(+)-FluoSTEP-EKAR | Genetically encoded FRET-based sensor for monitoring ERK activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence. |
| 181859 | pcDNA3.1(+)-cpPKN-mRuby2-GFP1-10 | Part of FluoSTEP-RhoA biosensor; must be expressed with GFP11-tagged RhoA plasmid (Addgene #181860) or in GFP11-RhoA gene-edited cells. |
| 181860 | pcDNA3.1(+)-GFP11-RhoA | Part of FluoSTEP-RhoA biosensor; co-express with cpPKN-mRuby2-GFP1-10 (Addgene #181859). |
| 181861 | pcDNA3.1(+)-FluoSTEP-AKAR (mScarlet-I) | FluoSTEP-AKAR using mScarlet-I as the FRET acceptor. |
| 181862 | pcDNA3-FluoSTEP-AKAR (C/Y) | FluoSTEP-AKAR using split CFP and cpVenus(E172) as the FRET donor and acceptor. Must pair with GFP11-tagged POI to reconstitute donor fluorescence. |
| 181863 | Lyn-FRB-GFP11 | Plasma membrane-localized GFP11; for plasma membrane targeting of FluoSTEPs. |
| 181966 | pEGFP-GFP11-Actin | Human beta actin tagged with GFP11. |
| 181967 | pcDNA3-GFP11(x7)-Actin | Human beta actin tagged with 7 copies of GFP11. |