zapERtrap: A light-regulated ER release system reveals unexpected neuronal trafficking pathways.

Bourke AM, Schwartz SL, Bowen AB, Kleinjan MS, Winborn CS, Kareemo DJ, Gutnick A, Schwarz TL, Kennedy MJ
J Cell Biol. 2021 Sep 6;220(9). pii: 212461. doi: 10.1083/jcb.202103186. Epub 2021 Jul 9. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

Showing 1 to 7 of 7 entries

Show entries

Search table:

ID	Plasmid	Purpose
173021	2xFKBP-smFP(myc)-KDEL	FKBP anchor for retaining DHFR-fused cargoes in the ER in the presence of zapalog. Encodes ER retrieval motif (KDEL) at C-terminus of spaghetti monster FP (myc, non-fluorescent). IRES following ORF.
173013	TfR-mCh-DHFR	For light regulated release of transferrin receptor (TfR) from the endoplasmic reticulum. Driven by CMV promoter.
173012	TfR-moxGFP-DHFR	For light regulated release of transferrin receptor (TfR) from the endoplasmic reticulum. Driven by CMV promoter. Fused to oxidation resistant version of GFP (moxGFP).
173010	2xFKBP-mCh-KDEL	FKBP anchor for retaining DHFR-fused cargoes in the ER in the presence of zapalog. Encodes ER retrieval motif (KDEL) at C-terminus of mCherry. Contains IRES following ORF for cloning cargo.
173009	DHFR-mNeon-GluA1	For light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Intracellular trafficking can be better visualized with mNeon.
173008	DHFR-GFP-th-GluA1	For light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Thrombin site is included between GFP and GluA1.
173007	DHFR-GFP-th-NL1	For light regulated release of neuroligin 1 from the endoplasmic reticulum. Driven by synapsin promoter. Thrombin site is included between GFP and NL1.