zapERtrap: A light-regulated ER release system reveals unexpected neuronal trafficking pathways.
Bourke AM, Schwartz SL, Bowen AB, Kleinjan MS, Winborn CS, Kareemo DJ, Gutnick A, Schwarz TL, Kennedy MJ
J Cell Biol. 2021 Sep 6;220(9). pii: 212461. doi: 10.1083/jcb.202103186. Epub 2021 Jul 9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 173007 | DHFR-GFP-th-NL1 | For light regulated release of neuroligin 1 from the endoplasmic reticulum. Driven by synapsin promoter. Thrombin site is included between GFP and NL1. |
| 173008 | DHFR-GFP-th-GluA1 | For light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Thrombin site is included between GFP and GluA1. |
| 173009 | DHFR-mNeon-GluA1 | For light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Intracellular trafficking can be better visualized with mNeon. |
| 173010 | 2xFKBP-mCh-KDEL | FKBP anchor for retaining DHFR-fused cargoes in the ER in the presence of zapalog. Encodes ER retrieval motif (KDEL) at C-terminus of mCherry. Contains IRES following ORF for cloning cargo. |
| 173012 | TfR-moxGFP-DHFR | For light regulated release of transferrin receptor (TfR) from the endoplasmic reticulum. Driven by CMV promoter. Fused to oxidation resistant version of GFP (moxGFP). |
| 173013 | TfR-mCh-DHFR | For light regulated release of transferrin receptor (TfR) from the endoplasmic reticulum. Driven by CMV promoter. |
| 173021 | 2xFKBP-smFP(myc)-KDEL | FKBP anchor for retaining DHFR-fused cargoes in the ER in the presence of zapalog. Encodes ER retrieval motif (KDEL) at C-terminus of spaghetti monster FP (myc, non-fluorescent). IRES following ORF. |