Comprehensive split TEV based protein-protein interaction screening reveals TAOK2 as a key modulator of Hippo signalling to limit growth.
Ma X, Mandausch FJ, Wu Y, Sahoo VK, Ma W, Leoni G, Hostiuc M, Wintgens JP, Qiu J, Kannaiyan N, Rossner MJ, Wehr MC
Cell Signal. 2024 Jan;113:110917. doi: 10.1016/j.cellsig.2023.110917. Epub 2023 Oct 7.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 172979 | pINDUCER21-puro_TAOK2 | doxycycline-inducible expression of TAOK2 |
| 172980 | pINDUCER21-puro_TAOK2-D151A | doxycycline-inducible expression of kinase-dead TAOK2 |
| 172981 | pINDUCER21-puro_Gateway-3xFLAG | Gateway Destination vector for doxycycline-inducible expression of ORF |
| 172982 | pLV_hU6-sgRNA_hUbC-dCas9-ZIM3-KRAB-T2a-PuroR | control & cloning vector for CRISPRi expressing dead Cas9-ZIM3-KRAB fusion |
| 172983 | pLV_dCas9-ZIM3-KRAB_sgTAOK2i_#1 | CRISPRi for TAOK2 |
| 172984 | pLV_dCas9-ZIM3-KRAB_sgTAOK2i_#2 | CRISPRi for TAOK2 |
| 172985 | pLV_dCas9-ZIM3-KRAB_sgTAOK2i_#3 | CRISPRi for TAOK2 |
| 172986 | pCMV7.1_3xFLAG-LATS1 | constitutive expression of FLAG-tagged LATS1 |
| 172987 | pCMV7.1_3xFLAG-LATS2 | constitutive expression of FLAG-tagged LATS2 |
| 172988 | pCMV7.1_3xFLAG-MOB1A | constitutive expression of FLAG-tagged MOB1A |
| 172989 | pCMV7.1_3xFLAG-STK4 | constitutive expression of FLAG-tagged STK4 |
| 172990 | pCMV7.1_3xFLAG-STK3 | constitutive expression of FLAG-tagged STK3 |
| 172991 | pCMV7.1_3xFLAG-TAOK1 | constitutive expression of FLAG-tagged TAOK1 |
| 172992 | pCMV7.1_3xFLAG-TAOK2 | constitutive expression of FLAG-tagged TAOK2 |
| 172993 | pCMV7.1_3xFLAG-TAOK3 | constitutive expression of FLAG-tagged TAOK3 |
| 172994 | pCMV7.1_3xFLAG-WWC1 | constitutive expression of FLAG-tagged WWC1 |
| 172995 | pCMV7.1_3xFLAG-WWC2 | constitutive expression of FLAG-tagged WWC2 |
| 172996 | pCMV7.1_3xFLAG-WWC3 | constitutive expression of FLAG-tagged WWC3 |
| 172997 | pcDNA3.1_nV5-AMOT (p130) | constitutive expression of V5-tagged AMOT (p130) |
| 172998 | pcDNA3.1_nV5-AMOTL1 | constitutive expression of V5-tagged AMOTL1 |
| 172999 | pcDNA3.1_nV5-AMOTL2 | constitutive expression of V5-tagged AMOTL2 |
| 173000 | pcDNA3.1_nV5-TAOK2 | constitutive expression of V5-tagged TAOK2 |
| 173001 | pcDNA3.1_FLAG-YAP1 | constitutive expression of FLAG-tagged YAP1 |
| 173002 | pLenti6_YAP1-V5 | constitutive expression of V5-tagged YAP1 |
| 173003 | pAAV_18xTEAD_luc2 | firefly luciferase reporter vector with 18x clustered TEAD binding sites |
| 173004 | pAAV_12xTEAD_luc2 | firefly luciferase reporter vector with 12x clustered TEAD binding sites |
| 173005 | pAAV_8xTEAD_luc2 | firefly luciferase reporter vector with 8x clustered TEAD binding sites |
| 173006 | p10xUAS-luc2 | firefly luciferase reporter vector with 10x clustered UAS binding sites |
| 209771 | pLV_dCas9-KRAB_sgSTK3i_#1 (PuroR) | CRISPRi for STK3 |
| 209772 | pLV_dCas9-KRAB_sgSTK3i_#2 (PuroR) | CRISPRi for STK3 |
| 209773 | pLV_dCas9-KRAB_sgSTK4i_#1 (PuroR) | CRISPRi for STK4 |
| 209774 | pLV_dCas9-KRAB_sgSTK4i_#2 (PuroR) | CRISPRi for STK4 |
| 209775 | pLV-NeoR_dCas9-Sniper-KRAB_sgSTK3i_#2 (NeoR) | CRISPRi for STK3 |
| 209776 | pLenti7-3_EF1ap-mRuby2_hU6p_sgSTK4i_#1 (BlasR) | CRISPRi for STK4 |
| 209777 | pINDUCER21-puro_TAOK1 | doxycycline-inducible expression of TAOK1 |
| 209778 | pINDUCER21-puro_TAOK3 | doxycycline-inducible expression of TAOK3 |