Engineering designer beta cells with a CRISPR-Cas9 conjugation platform.
Lim D, Sreekanth V, Cox KJ, Law BK, Wagner BK, Karp JM, Choudhary A
Nat Commun. 2020 Aug 13;11(1):4043. doi: 10.1038/s41467-020-17725-0.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 206251 | pDL17_Cas9-His Δcys M1C | Bacterial expression of SpCas9 Δcys M1C variant under T7 promoter |
| 206283 | pDL22_Cas9-His Δcys E532C | Bacterial expression of Cas9-His Δcys E532C variant under T7 promoter |
| 206284 | pDL23_Cas9-His Δcys E945C | Bacterial expression of Cas9-His Δcys E945C variant under T7 promoter |
| 206285 | pDL24_Cas9-His Δcys E1026C | Bacterial expression of Cas9-His Δcys E1026C variant under T7 promoter |
| 206286 | pDL25_Cas9-His Δcys E1207C | Bacterial expression of Cas9-His Δcys E1027C variant under T7 promoter |
| 206287 | pDL26_Cas9-His Δcys M1C/E532C | Bacterial expression of SpCas9 Δcys M1C and E532C variant under T7 promoter |
| 206288 | pDL27_Cas9-His Δcys E532C/E945C | Bacterial expression of SpCas9 Δcys E532C and E945C variant under T7 promoter |
| 206289 | pDL28_Cas9-His Δcys E532C/E1207C | Bacterial expression of SpCas9 Δcys E532C and E1027C variant under T7 promoter |