Wnt-inducible Lrp6-APEX2 interacting proteins identify ESCRT machinery and Trk-fused gene as components of the Wnt signaling pathway.
Colozza G, Jami-Alahmadi Y, Dsouza A, Tejeda-Munoz N, Albrecht LV, Sosa EA, Wohlschlegel JA, De Robertis EM
Sci Rep. 2020 Dec 9;10(1):21555. doi: 10.1038/s41598-020-78019-5.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
180142 | pCS2 LRP6-APEX2-Ires-eGFP-PAC | used to biotinylate targets that are recruited near the receptor during Wnt signaling at different time periods |
180143 | pCS2 LRP6-eGFP | to make movies of LRP6 vesicles |
180144 | pCS2-LRP6-3XFlag | flag tagged LRP6 |