Engineered mini H1 promoters with dedicated RNA Polymerase II or III activity.
Gao Z, van der Velden YU, Fan M, van der Linden CA, Vink M, Herrera-Carrillo E, Berkhout B
J Biol Chem. 2020 Nov 5. pii: S0021-9258(20)00012-5. doi: 10.1074/jbc.RA120.015386.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 193663 | H1.99.luc | Expresses a truncated Polymerase III H1 promoter of 99 nucleotides (H1-99) |
| 193664 | H1.99.M.luc | Expresses a truncated Polymerase III H1 promoter of 99 nucleotides (H1-99) with the TATA box mutated |
| 193704 | H1.224.luc | Expresses a truncated PolII H1 promoter (H1.244) |
| 193705 | H1.224.M.luc | Expresses a truncated PolIII H1 promoter of 224 nucleotides (H1.224) with the TATA box mutated |
| 193706 | H1.M10.luc | Expressed a H1-core mutant by introducing multiple 7SK elements (PSE, spacer, and TATA box) |
| 193707 | H1.M11.luc | Expressed a H1-core mutant by introducing multiple 7SK elements (octamer, PSE, spacer, and TATA box) |