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Engineering of ultraID, a compact and hyperactive enzyme for proximity-dependent biotinylation in living cells.

Kubitz L, Bitsch S, Zhao X, Schmitt K, Deweid L, Roehrig A, Barazzone EC, Valerius O, Kolmar H, Bethune J
Commun Biol. 2022 Jul 4;5(1):657. doi: 10.1038/s42003-022-03604-5. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
172878pSF3-ultraIDexpresses ultraID in mammalian cells with the tet-on or tet-off system
172879pet-15b-ultraIDexpresses ultraID in E. coli
172880pSF3-microIDexpresses microID in mammalian cells with the tet-on or tet-off system
172881pet-15b-microIDexpresses microID in E. coli

Antibodies from Article