Engineering of a fluorescent chemogenetic reporter with tunable color for advanced live-cell imaging.
Benaissa H, Ounoughi K, Aujard I, Fischer E, Goiame R, Nguyen J, Tebo AG, Li C, Le Saux T, Bertolin G, Tramier M, Danglot L, Pietrancosta N, Morin X, Jullien L, Gautier A
Nat Commun. 2021 Nov 30;12(1):6989. doi: 10.1038/s41467-021-27334-0.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 130820 | pAG498 MAP4-iFAST | Expresses MAP4-iFAST in mammalian cells (microtubule labeling) |
| 130821 | pAG470 LifeAct-iFAST | Expresses LifeAct-iFAST in mammalian cells (actin labeling) |
| 172861 | pAG654 pFAST | Expresses pFAST in mammalian cells |
| 172862 | pAG657 H2B-pFAST | Expresses pFAST fused to zebrafish histone H2B in mammalian cells |
| 172863 | pAG660 lyn11-pFAST | expresses lyn11-pFAST in mammalian cells (inner membrane labeling) |
| 172865 | pAG665 MAP4-pFAST | Expresses MAP4-pFAST in mammalian cells (microtubule labeling) |
| 172866 | pAG668 lifeAct-pFAST | Expresses LifeAct-pFAST in mammalian cells (actin labeling) |
| 172867 | pAG671 mito-pFAST | Expresses mitochondrial targeting domain-pFAST in mammalian cells |
| 172868 | pAG876 pDisplay-pFAST | expresses pFAST at the surface of mammalian cells |