A versatile genetic engineering toolkit for E. coli based on CRISPR-prime editing.
Tong Y, Jorgensen TS, Whitford CM, Weber T, Lee SY
Nat Commun. 2021 Sep 1;12(1):5206. doi: 10.1038/s41467-021-25541-3.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 172715 | pCRISPR-PE-bacteria | Expression of an E. coli codon optimized fusion protein of Cas9n-linker-M-MLV2 for prime editing |
| 172716 | pPEgRNA | Delivery of guide RNA for prime editing |
| 172717 | pnsgRNA | Delivery of guide RNA for prime editing |
| 172718 | pCDF-GFPplus | Expression of a fast folding GFP variant GFP plus |