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Global and precise identification of functional miRNA targets in mESCs by integrative analysis.

Schaefer M, Nabih A, Spies D, Hermes V, Bodak M, Wischnewski H, Stalder P, Ngondo RP, Liechti LA, Sajic T, Aebersold R, Gatfield D, Ciaudo C
EMBO Rep. 2022 Jul 28:e54762. doi: 10.15252/embr.202254762. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
172709pX458_miR-290-295KO-gRNA1Cas9 with 5' gRNA for paired CRISPR KO of miR-290-295 cluster
172710pX458_miR-290-295KO-gRNA2Cas9 with 3' gRNA for paired CRISPR KO of miR-290-295 cluster
172711pX458_miR-290-295KO-gRNA3Cas9 with 5' gRNA for paired CRISPR KO of miR-290-295 cluster
185054TFAP4_BHLH_5-1Deletion of BHLH domain of mouse TFAP4 in combination with TFAP4_BHLH_3-2
185055TFAP4_BHLH_3-2Deletion of BHLH domain of mouse TFAP4 in combination with TFAP4_BHLH_5-1 or TFAP4_BHLH_5-2
185056TFAP4_BHLH_5-2Deletion of BHLH domain of mouse TFAP4 in combination with TFAP4_BHLH_3-2

Antibodies from Article