Quantitative imaging of RNA polymerase II activity in plants reveals the single-cell basis of tissue-wide transcriptional dynamics.
Alamos S, Reimer A, Niyogi KK, Garcia HG
Nat Plants. 2021 Aug;7(8):1037-1049. doi: 10.1038/s41477-021-00976-0. Epub 2021 Aug 9.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
161003 | UPG | ubiquitous expression of PCP-GFP |
161004 | UPmCh | ubiquitous expression of PCP-mCherry |
161005 | UMsfG | ubiquitous expression of MCP-sfGFP |
161006 | AL13Rb | PP7-tagged reporter without a promoter |
161007 | AL12R | PP7-tagged reporter without a promoter |
161008 | AL13Rb-35S | PP7-tagged reporter driven by the 35S promoter |
161009 | AL13Rb-GAPC2 | PP7-tagged reporter driven by the Arabidopsis GAPC2 promoter |
161010 | AL12R-HSP70 | PP7-tagged reporter driven by the Arabidopsis HSP70 promoter |
161011 | HSP70-pp7i-mCh-UPG | C-terminal mCherry fusion to Arabidopsis HSP70 with intronic PP7 |
161012 | AL13Rb-HsfA2 | PP7-tagged reporter driven by the Arabidopsis HsfA2 promoter |
161013 | AL12R-EF-Tu | PP7-tagged reporter driven by the Arabidopsis EF-Tu promoter |
161014 | AL12R-HSP101 | PP7-tagged reporter driven by the Arabidopsis HSP101 promoter |
161015 | UtB2N7 | ubiquitously expressed nuclear localized tagBFP2 |
161016 | UBC1cer60G | ubiquitously weakly expressed 60-meric GFP nanocage |
161017 | UBC1cer120G | ubiquitously weakly expressed 120-meric GFP nanocage |
172699 | UBC1cer40GEM | Ubiquitous expression of 60meric GEM monomer |