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Quantitative imaging of RNA polymerase II activity in plants reveals the single-cell basis of tissue-wide transcriptional dynamics.

Alamos S, Reimer A, Niyogi KK, Garcia HG
Nat Plants. 2021 Aug;7(8):1037-1049. doi: 10.1038/s41477-021-00976-0. Epub 2021 Aug 9. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
161003UPGubiquitous expression of PCP-GFP
161004UPmChubiquitous expression of PCP-mCherry
161005UMsfGubiquitous expression of MCP-sfGFP
161006AL13RbPP7-tagged reporter without a promoter
161007AL12RPP7-tagged reporter without a promoter
161008AL13Rb-35SPP7-tagged reporter driven by the 35S promoter
161009AL13Rb-GAPC2PP7-tagged reporter driven by the Arabidopsis GAPC2 promoter
161010AL12R-HSP70PP7-tagged reporter driven by the Arabidopsis HSP70 promoter
161011HSP70-pp7i-mCh-UPGC-terminal mCherry fusion to Arabidopsis HSP70 with intronic PP7
161012AL13Rb-HsfA2PP7-tagged reporter driven by the Arabidopsis HsfA2 promoter
161013AL12R-EF-TuPP7-tagged reporter driven by the Arabidopsis EF-Tu promoter
161014AL12R-HSP101PP7-tagged reporter driven by the Arabidopsis HSP101 promoter
161015UtB2N7ubiquitously expressed nuclear localized tagBFP2
161016UBC1cer60Gubiquitously weakly expressed 60-meric GFP nanocage
161017UBC1cer120Gubiquitously weakly expressed 120-meric GFP nanocage
172699UBC1cer40GEMUbiquitous expression of 60meric GEM monomer

Antibodies from Article