Enhancing prime editing by Csy4-mediated processing of pegRNA.
Liu Y, Yang G, Huang S, Li X, Wang X, Li G, Chi T, Chen Y, Huang X, Wang X
Cell Res. 2021 Jun 8. pii: 10.1038/s41422-021-00520-x. doi: 10.1038/s41422-021-00520-x.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 172667 | pCMV-Csy4-PE2 | Enhanced prime editing system in mammalian cells. This plasmid is used by PE3 and PE3b |
| 172668 | PGL3-U6-ALDOB_pegRNA-Csy4RS-nick_sgRNA-EGFP | For expression of transcript containing pegRNA and nick-sgRNA targeting human ALDOB gene from the U6 promoter with pegRNA flanked by Csy4 recognition site |
| 172669 | PGL3-U6-RNF2_pegRNA-Csy4RS-nick_sgRNA-EGFP | For expression of transcript containing pegRNA and nick-sgRNA targeting human RNF2 gene from the U6 promoter with pegRNA flanked by Csy4 recognition site |
| 172670 | PGL3-U6-SDHB_pegRNA-Csy4RS-nick_sgRNA-EGFP | For expression of transcript containing pegRNA and nick-sgRNA targeting human SDHB gene from the U6 promoter with pegRNA flanked by Csy4 recognition site |