Precise genomic deletions using paired prime editing.
Choi J, Chen W, Suiter CC, Lee C, Chardon FM, Yang W, Leith A, Daza RM, Martin B, Shendure J
Nat Biotechnol. 2021 Oct 14. pii: 10.1038/s41587-021-01025-z. doi: 10.1038/s41587-021-01025-z.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 172655 | PB-HD118 | Expressing paired pegRNAs from human U6 and H1 promoters to make 118-bp deletion on HPRT1 gene |
| 172656 | PB-HD252 | Expressing paired pegRNAs from human U6 and H1 promoters to make 252-bp deletion on HPRT1 gene |
| 172657 | pU6nH1-HD10kb | Expressing paired pegRNAs from human U6 and H1 promoters to make 10204-bp deletion on HPRT1 gene |
| 172658 | Lentiguide-Blast-eNMU710 | Expressing paired pegRNAs from human U6 and H1 promoters to make 710-bp deletion on e-NMU locus |