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A selectable all-in-one CRISPR prime editing piggyBac transposon allows for highly efficient gene editing in human cell lines.

Eggenschwiler R, Gschwendtberger T, Felski C, Jahn C, Langer F, Sterneckert J, Hermann A, Luhmann J, Steinemann D, Haase A, Martin U, Petri S, Cantz T
Sci Rep. 2021 Nov 12;11(1):22154. doi: 10.1038/s41598-021-01689-2. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
162796PB-PEAll-in-one prime editor piggyBac transposon
162797PB-PE VQRAll-in-one prime editor piggyBac transposon, VQR variant
172652PB-PE xCas9All-in-one prime editor piggyBac transposon (xCas9)
174554PB-PE NGAll-in-one prime editor piggyBac transposon, NG variant
179081PB-PE SpGAll-in-one prime editor piggyBac transposon, SpG variant
179082PB-PE SpRYAll-in-one prime editor piggyBac transposon, SpRY variant
187893PB-PEmaxAll-in-one prime editor piggyBac transposon with PEmax
187894PB-PEmax NGAll-in-one prime editor piggyBac transposon with PEmax, NG variant
187895PB-PEmax SpGAll-in-one prime editor piggyBac transposon with PEmax, SpG variant

Antibodies from Article