Comparative analysis of MACROD1, MACROD2 and TARG1 expression, localisation and interactome.
Zaja R, Aydin G, Lippok BE, Feederle R, Luscher B, Feijs KLH
Sci Rep. 2020 May 19;10(1):8286. doi: 10.1038/s41598-020-64623-y.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 172572 | pDONR/zeo-MACROD1 | For gateway cloning of N-terminal tagged MACROD1 constructs. |
| 172573 | pDONR/zeo-MACROD2 | For gateway cloning of N-terminal tagged MACROD2 constructs. |
| 172574 | pDONR/zeo-TARG1 | For gateway cloning of N-terminal tagged TARG1 constructs. |
| 172577 | pDONR221-TARG1 | For gateway cloning of C-terminal tagged TARG1 constructs. |
| 172578 | pcDNA5/FRT/TO/MACROD1 | For generation of FlpIn lines with untagged MACROD1 |
| 172579 | pcDNA5/FRT/TO/MACROD2 | For generation of FlpIn lines with untagged MACROD2 |
| 172580 | pcDNA5/FRT/TO/TARG1 | For generation of FlpIn lines with untagged TARG1 |
| 172581 | pcDNA3.1-MCS-BirA(R118G)-HA-MACROD1 | For transient expression of C-terminal BirA-tagged MACROD1 |
| 172582 | pcDNA3.1-MCS-BirA(R118G)-HA-MACROD2 | For transient expression of C-terminal BirA-tagged MACROD2 |
| 172583 | pcDNA3.1-MCS-BirA(R118G)-HA-TARG1 | For transient expression of C-terminal BirA-tagged TARG1 |
| 172584 | pDEST-EGFP-MACROD1 | For transient expression of N-terminal GFP-tagged MACROD1 |
| 172585 | pDEST-EGFP-MACROD2 | For transient expression of N-terminal GFP-tagged MACROD2 |
| 172586 | pDEST-EGFP-TARG1 | For transient expression of N-terminal GFP-tagged TARG1 |
| 172587 | pcDNA3-mRuby2-MACROD1 | For transient expression of C-terminal mRuby2-tagged MACROD1 |
| 172588 | pcDNA3-mRuby2-MACROD1_d77 | For transient expression of C-terminal mRuby2-tagged MACROD1del1-77 |
| 172594 | pDEST17-TARG1 | For purification (from E.coli) of His-tagged TARG1 |