Super-resolved 3D tracking of cargo transport through nuclear pore complexes.
Chowdhury R, Sau A, Musser SM
Nat Cell Biol. 2022 Jan;24(1):112-122. doi: 10.1038/s41556-021-00815-6. Epub 2022 Jan 10.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 172489 | pM9-BetaGal-8C | Plasmid encoding E. coli BetaGalactosidase with an N-terminal M9 nuclear localization sequence |
| 172490 | pET21b-LaG9(S151C) | Encodes the anti-GFP LaG-9 nanobody with a cysteine near the C-terminus, LaG-9(S151C). |
| 176151 | pNLS-2xBFP(4C) | Encodes NLS-2xBFP(4C), which is a tandem BFP with an N-terminal SV40 NLS. The protein has 4 solvent accessible cysteines for labeling with maleimide dyes. |