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Optogenetic control of receptors reveals distinct roles for actin- and Cdc42-dependent negative signals in chemotactic signal processing.

Bell GRR, Rincon E, Akdogan E, Collins SR
Nat Commun. 2021 Nov 16;12(1):6148. doi: 10.1038/s41467-021-26371-z. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
172472pLV_PP1-mCitrineMammalian expression of a human codon-optimized version of the optogenetic GPCR parapinopsina (PP1) from zebrafish fused to mCitrine. Also contains an N-terminal prolactin signal sequence.
172473pPB_Cdc42-TomKat-sensor-CAAXPiggybac vector for mammalian expression of TomKat (tdTomato-tdKatushka) FRET sensor for Cdc42 activity anchored to the membrane with the polybasic and CAAX motif from Kras.

Antibodies from Article