Genome-wide base editor screen identifies regulators of protein abundance in yeast.
Schubert OT, Bloom JS, Sadhu MJ, Kruglyak L
Elife. 2022 Nov 3;11. pii: 79525. doi: 10.7554/eLife.79525.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
172409 | pGal-BE3 | Yeast plasmid encoding the third-generation CRISPR base editor BE3 (Komor et al., Nature 2016) under a galactose-inducible promoter |
172517 | pgRNA-backbone | Yeast plasmid encoding a 2-kb gRNA-placeholder flanked by BsmBI/Esp3I restriction sites to facilitate gRNA insertion |