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Genome-wide base editor screen identifies regulators of protein abundance in yeast.

Schubert OT, Bloom JS, Sadhu MJ, Kruglyak L
Elife. 2022 Nov 3;11. pii: 79525. doi: 10.7554/eLife.79525. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
172409pGal-BE3Yeast plasmid encoding the third-generation CRISPR base editor BE3 (Komor et al., Nature 2016) under a galactose-inducible promoter
172517pgRNA-backboneYeast plasmid encoding a 2-kb gRNA-placeholder flanked by BsmBI/Esp3I restriction sites to facilitate gRNA insertion

Antibodies from Article