Background-suppressed live visualization of genomic loci with an improved CRISPR system based on a split fluorophore.
Chaudhary N, Nho SH, Cho H, Gantumur N, Ra JS, Myung K, Kim H
Genome Res. 2020 Sep;30(9):1306-1316. doi: 10.1101/gr.260018.119. Epub 2020 Sep 4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 172408 | pM423 | Expresses sfGFP (1-10) and MCP-GFP11 |
| 173173 | pM80 | HA-dCas9 |
| 173174 | pM378 | Expresses C9-1 gRNA-12xMBS from hU6 promoter |
| 173175 | pM355 | (Empty Backbone) Expresses gRNA-12xMBS from hU6 promoter |
| 173178 | pM370 | (Empty Backbone) gRNA-12xMBS |
| 173179 | pM201 | dCas9-24xGCN4 |
| 173215 | pM159 | Expresses dCas9-sfGFP10 |
| 173216 | pM214 | Expresses MCP-mCherry-GFP11-GB1 |
| 173217 | pM215A | Expresses ScFV-GCN4-sFGFP10M2-GB1 |
| 173218 | pM198 | Expresses OptGFP(1-9)-GB1 |
| 173221 | pM162 | Expresses ScFV-GCN4-GFP10M2-GB1-T2A-OptGFP(1-9)-GB1 and Puro-T2A-MCP-mCherry-GFP11-GB1 |