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Fast, high-throughput production of improved rabies viral vectors for specific, efficient and versatile transsynaptic retrograde labeling.

Sumser A, Joesch M, Jonas P, Ben-Simon Y
Elife. 2022 Aug 30;11:e79848. doi: 10.7554/eLife.79848. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
172359pAAV-DIO-Ef1a-TVA-2A-oGTargeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling specifically from cre-expressing neurons
172360pAAV-DIO-Ef1a-TVA-2A-N2cGTargeting envA-pseodotyped RVdG vectors for specific retrograde labeling specifically from cre-expressing neurons
172361pAAV-FRT-EF1a-TVA-2A-N2cGTargeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from Flp-expressing neurons
172362pAAV-DIO-CaMKII-TVA-P2A-N2cGTargeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from cre-expressing excitatory cortical neurons
172363pAAV-SEO-CaMKII-TVA-P2A-N2cGTargeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from excitatory cortical neurons which do not express cre-recombinase
172364pAAV-mDLX-TVA-2A-N2cGTargeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from inhibitory neurons
172365pAAV-DIO-mDLX-TVA-2A-N2cGTargeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from cre-expressing inhibitory neurons
172366MMLV-CAG-TVA-IRES-PuroGeneration of TVA-expressing stable cell lines, resistant to the antibiotic Puromycin
172367MMLV-CAG-SADB19_oG-IRES-PuroGeneration of stable cell lines expressing the rabies optimized glycoprotein (oG), resistant to the antibiotic Puromycin
172368pLV-EF1a-N2c_envA-IRES-NeoGeneration of envA-expressing stable cell lines, resistant to the antibiotic Neomycin (Gentamicin/G418)
172369MMLV-CAG-G_oT7pol-IRES-BSDGeneration of oT7pol-expressing stable cell lines, resistant to the antibiotic Blasticidin
172370CVS-N2c-tdTomato-ChIEFProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.
172371CVS-N2c-EGFP-ChIEFProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.
172372CVS-N2c-EGFP-iCreProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.
172373CVS-N2c-EGFP-FlpOProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.
172374CVS-N2c-tdTomato-iCreProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.
172375CVS-N2c-tdTomato-FlpOProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.
172376CVS-N2c-mTurquoiseProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.
172377CVS-N2c-E2_CrimsonProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.
172378CVS-N2c-nl.mCherry-FlpOProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.
172379CVS-N2c-nl.EGFP-FlpOProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.
172380CVS-N2c-nl.EGFP-SypGFPProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.
172381CVS-N2c-SypRFPProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.
172382CVS-N2c-nl.EGFP-tdTomatoProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.
172383CVS-N2c-EYFPProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.
172384CVS-N2c-mCitrineProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.
172385CVS-N2c-nl.mCherry-GCaMP7sProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.
172386CVS-N2c-nl.EGFP-jRGECO1aProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.
172387CVS-N2c-jGCaMP8fProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.
172388CVS-N2c-jGCaMP8mProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.
172389CVS-N2c-jGCaMP8sProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

Antibodies from Article