dFLASH; dual FLuorescent transcription factor Activity Sensor for Histone integrated live-cell reporting and high-content screening
Allen TP, Roennfeldt AE, Reckdharajkumar M, Liu M, Quinn RJ, Russell DL, Peet DJ, Whitelaw ML, Bersten DC
bioRxiv 2023.11.21.568191
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 172326 | pLV-REPORT(EF1a) | LV REPORT containing minimal promoter driving monomeric nuclear Tomato followed by an EF1a promoter driving nuclear d2eGFP E2A Hygromycin |
| 172327 | pLV-REPORT(EF1a)-TTN | LV REPORT containing minimal promoter driving monomeric nuclear Tomato 2a HSVtk 2a Neo followed by an EF1a promoter driving nuclear d2eGFP E2A Hygromycin |
| 172328 | pLV-REPORT(PGK) | LV REPORT containing minimal promoter driving monomeric nuclear Tomato 2a HSVtk 2a Neo followed by an PGK promoter driving nuclear d2eGFP E2A Hygromycin |
| 172330 | pLV-REPORT(PGK/CMV) | LV REPORT containing minimal promoter driving monomeric nuclear Tomato 2a HSVtk 2a Neo followed by an PGK/CMV promoter driving nuclear d2eGFP E2A Hygromycin |
| 172334 | pLV-REPORT(PGK)-12xHRE | hypoxic responsive reporter |
| 172335 | pLV-REPORT(PGK/CMV)-12xHRE | hypoxic responsive reporter |
| 172336 | pLV-REPORT(PGK/CMV)-GRE | Gal4 Response element |
| 172339 | pLV-REPORT(EF1a no GFP) | mnucTomato |
| 172342 | pLV-REPORT(EF1a no GFP) mnuctdTomato | mnuctdTomato |
| 172352 | pLV-REPORT(EF1a)TetR | Tet Repressor reporter |
| 207169 | pLV-TET2Puro-gal4DBD-miniVPR-HA | Dox Inducible Expression Plasmid for gal4DBD-miniVPR-HA |
| 207171 | pEF-IRES-puro6 gal4DBD-HIFCAD myc tag | expression plasmid for Gal4DBD-HIF-CAD.myc expression |
| 207172 | pEF-IRES-puro6 +gal4DBDHIFCAD pGalO linker | expression plasmid for Gal4DBD-HIF-CAD expression GalO linker |
| 207173 | pLV-tet2puro-gal4DBD-HIFCAD | Dox Inducible Expression Plasmid for gal4DBD-HIF-CAD |
| 207174 | pENTR1a-CRISPRoffv2.1 | Gateway Cloning Vector for CRISPRoffv2.1 |
| 207175 | pLV-EgI-NeoR | Gateway compatible Lentiviral Expression Plasmid EF1a-IRES-NeoR |
| 207176 | pLV-EgI-BlastR | Gateway compatible Lentiviral Expression Plasmid EF1a-IRES-BlastR |
| 207177 | pLV-EgI-HygroR | Gateway compatible Lentiviral Expression Plasmid EF1a-IRES-HygroR |
| 207178 | pLV-EgI-ZeoR | Gateway compatible Lentiviral Expression Plasmid EF1a-IRES-ZeoR |
| 207179 | pLV-SFFVp-gI-BlastR | Gateway compatible Lentiviral Expression Plasmid SFFVp-IRES-BlastR |
| 207180 | pLV-EF1a-CRISPRoffv2.1-IRES-BlastR | Lentiviral Expression Plasmid for CRISPRoff2.1 driven by EF1a Promoter |
| 207181 | pLV-SFFVp-CRISPRoffv2.1-IRES-BlastR | Lentiviral Expression Plasmid for CRISPRoff2.1 driven by SFFV Promoter |
| 207182 | pLV-SV40p-gI-BlastR | Gateway compatible Lentiviral Expression Plasmid SV40p-IRES-BlastR |
| 208573 | pLV-REPORT(PGKCMV)-nucTomato-hygro | LV REPORT containing minimal promoter driving monomeric nuclear Tomato followed by an PGK/CMV promoter driving nuclear d2eGFP E2A Hygromycin |
| 208574 | pLV-REPORT(PGK/CMV)-GtwyA | LV REPORT containing minimal promoter (GtwyA inserted into MCS) driving monomeric nuclear Tomato followed by an PKG/CMV promoter driving nuclear d2eGFP E2A Hygromycin |
| 208575 | pLV-REPORT (PGK) 12xHRE-NanoLuc | hypoxic response element driven NanoLuc Lentiviral reporter |
| 208576 | pLV-REPORT (PGK) NanoLuc | Empty NanoLuc Lentiviral reporter |