CRISPR-based targeting of DNA methylation in Arabidopsis thaliana by a bacterial CG-specific DNA methyltransferase.
Ghoshal B, Picard CL, Vong B, Feng S, Jacobsen SE
Proc Natl Acad Sci U S A. 2021 Jun 8;118(23):e2125016118. doi: 10.1073/pnas.2125016118.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 172316 | pEG302_dCAS9-MQ1(Q147L)_g4+g10+g18 | CRISPR dCas9 directly fused to a variant of bacterial DNA methyltransferase MQ1 to target CG specific methylation to the FWA locus with three guide RNAs |
| 172317 | pEG302_dCAS9-MQ1(C141S,S317A)_g4+g10+g18 | CRISPR dCas9 directly fused to a catalytic inactive version of bacterial DNA methyltransferase MQ1 to target to the FWA locus with three guide RNAs |
| 172318 | pEG302_22aa_SunTag_MQ1(Q147L)_g4+g10+g18 | CRISPR dCas9 SunTag system to target a variant of bacterial DNA methyltransferase MQ1 to install CG specific methylation to the FWA locus with three guide RNAs |