A molecular beacon-based approach for live-cell imaging of RNA transcripts with minimal target engineering at the single-molecule level.
Chen M, Ma Z, Wu X, Mao S, Yang Y, Tan J, Krueger CJ, Chen AK
Sci Rep. 2017 May 8;7(1):1550. doi: 10.1038/s41598-017-01740-1.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
172281 | pEGFP-N1-1x | Expressing EGFP mRNA fused with 1 tandem repeat of a 50-base sequence |
172282 | pEGFP-N1-2x | Expressing EGFP mRNA fused with 2 tandem repeats of a 50-base sequence |
172283 | pEGFP-N1-4x | Expressing EGFP mRNA fused with 4 tandem repeats of a 50-base sequence |
172284 | pEGFP-N1-8x | Expressing EGFP mRNA fused with 8 tandem repeats of a 50-base sequence |