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A molecular beacon-based approach for live-cell imaging of RNA transcripts with minimal target engineering at the single-molecule level.

Chen M, Ma Z, Wu X, Mao S, Yang Y, Tan J, Krueger CJ, Chen AK
Sci Rep. 2017 May 8;7(1):1550. doi: 10.1038/s41598-017-01740-1. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
172281pEGFP-N1-1xExpressing EGFP mRNA fused with 1 tandem repeat of a 50-base sequence
172282pEGFP-N1-2xExpressing EGFP mRNA fused with 2 tandem repeats of a 50-base sequence
172283pEGFP-N1-4xExpressing EGFP mRNA fused with 4 tandem repeats of a 50-base sequence
172284pEGFP-N1-8xExpressing EGFP mRNA fused with 8 tandem repeats of a 50-base sequence

Antibodies from Article