Topological isolation of developmental regulators in mammalian genomes.
Wu HJ, Landshammer A, Stamenova EK, Bolondi A, Kretzmer H, Meissner A, Michor F
Nat Commun. 2021 Aug 12;12(1):4897. doi: 10.1038/s41467-021-24951-7.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 172221 | 2X_pX458_pSpCas9(BB)-2A-GFP | Cas9-2A-GFP expression vector bearing two (one additional) independent sgRNA cassettes. |
| 172224 | 2X_pX458_pSpCas9(BB)-2A-GFP_NANOG_bKO | Cas9-2A-GFP expression vector bearing two sgRNAs targeting the telomeric human NANOG CTCF-boundary. |
| 172225 | 2X_pX458_pSpCas9(BB)-2A-GFP_SOX17_bKO | Cas9-2A-GFP expression vector bearing two sgRNAs targeting the centromeric human SOX17 CTCF-boundary. |
| 172226 | PB-CAG-DD-3xFLAG-hSOX17-GS-TagBFP-BGHpA | Expression vector with Shield-1 stabilizable hSOX17 fused to TagBFP. |