Marker-Free Genome Engineering in Amycolatopsis Using the pSAM2 Site-Specific Recombination System.
Santos LDF, Caraty-Philippe L, Darbon E, Pernodet JL
Microorganisms. 2022 Apr 16;10(4). pii: microorganisms10040828. doi: 10.3390/microorganisms10040828.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 170766 | pEA01 | Utilisation of pSAM2 excision tool in Amycolatopsis spp |
| 172193 | pEA02 | Suicide vector carrying the site attL of the SSR system of pSAM2. This plasmid is integrated into the genome of Actinobacteria by HR when carrying a DNA fragment identical to a region of the genome. |
| 172194 | pEA03 | Suicide vector carrying the site attR of the SSR system of pSAM2. This plasmid is integrated into the genome of Actinobacteria by HR when carrying a DNA fragment identical to a region of the genome. |