Improved pyrrolysine biosynthesis through phage assisted non-continuous directed evolution of the complete pathway.
Ho JML, Miller CA, Smith KA, Mattia JR, Bennett MR
Nat Commun. 2021 Jun 24;12(1):3914. doi: 10.1038/s41467-021-24183-9.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 172141 | pJH19 | Accessory Plasmid for PACE of pylBCD variants |
| 172142 | pJH60 | Microscopy inclusion body assay |
| 172143 | pJH61 | Accessory Plasmid for PACE of pylBCD variants |
| 172144 | pJH76 | Accessory Plasmid for PACE of pylBCD variants |
| 172145 | pJH77 | Accessory Plasmid for PACE of pylBCD variants |
| 172146 | pJH123 | Microscopy inclusion body assay |
| 172147 | pJH126 | Microscopy inclusion body assay |
| 172148 | pJH190 | Accessory Plasmid for PACE of pylBCD variants |
| 172149 | pJH191 | Accessory Plasmid for PACE of pylBCD variants |
| 172150 | pJH202 | Accessory Plasmid for PACE of pylBCD variants |
| 172151 | pJH223 | pylBCD variant in pCDF backbone |
| 172152 | pJH224 | pylBCD variant in pCDF backbone |
| 172153 | pJH291 | Reporter plasmid for pylBCD variant in pCDF backbone |
| 172154 | pJH462 | pylBCD variant in pBAD backbone |
| 172155 | pJH463 | pylBCD variant in pBAD backbone |
| 172156 | pJH572 | Negative control pBAD backbone |
| 172157 | pJH573 | pylBCD variant in pBAD backbone |
| 172158 | pJH574 | pylBCD variant in pBAD backbone |
| 172159 | pJH575 | pylBCD variant in pBAD backbone |
| 172160 | pJH576 | pylBCD variant in pBAD backbone |
| 172161 | pJH577 | pylBCD variant in pBAD backbone |
| 172162 | pJH578 | pylBCD variant in pBAD backbone |
| 172163 | pJH579 | pylBCD variant in pBAD backbone |
| 172164 | pJH580 | pylBCD variant in pBAD backbone |
| 172165 | pJH581 | pylBCD variant in pBAD backbone |
| 172168 | pJH643 | Reporter plasmid for pylBCD variant in pBAD backbone |
| 172169 | pJH645 | Reporter plasmid for pylBCD variant in pBAD backbone |
| 172170 | pJH750 | Microscopy inclusion body assay |
| 172171 | pJH751 | Microscopy inclusion body assay |
| 172172 | pJH767 | PylB overexpression and purification |
| 172173 | pJH768v2 | PylB overexpression and purification |
| 172174 | pJH769v2 | PylB overexpression and purification |
| 172175 | pJH857 | PylB overexpression and purification |
| 172176 | pJH858 | PylB overexpression and purification |
| 172177 | pJH859 | PylB overexpression and purification |